解決方案

關鍵技術


RNA 建庫專家

SENSE mRNA-Seq Library Prep Kit V2

SENSE is a complete strand-specific mRNA-Seq library prep kit for accurate gene expression profiling, transcriptome sequencing, discovery and quantification of antisense transcripts and overlapping genes.

Analysis of poly(A) RNA

 

Superior Strand-Specificity

The strand-displacement stop/ligation technology used in SENSE generates fewer antisense artifacts which can be produced by template-switching in protocols which utilize RNA or cDNA fragmentation. This results in exceptional (>99.9 %) strand-specificity and reduced experimental noise, enabling the detection and quantification of antisense transcripts with high confidence.


Table | Summary of the sequencing results comparing SEQC sample A (mixture of Universal Human Reference RNA with ERCC Exfold Spike-In Mix 1) with SENSE mRNA-Seq and conventional RNA-Seq.

 

Superior gene body coverage

  • More evenly accumulated coverage of 3' and 5' transcript ends