SENSE mRNA-Seq Library Prep Kit V2
SENSE is a complete strand-specific mRNA-Seq library prep kit for accurate gene expression profiling, transcriptome sequencing, discovery and quantification of antisense transcripts and overlapping genes.
Analysis of poly(A) RNA
The strand-displacement stop/ligation technology used in SENSE generates fewer antisense artifacts which can be produced by template-switching in protocols which utilize RNA or cDNA fragmentation. This results in exceptional (>99.9 %) strand-specificity and reduced experimental noise, enabling the detection and quantification of antisense transcripts with high confidence.
Table | Summary of the sequencing results comparing SEQC sample A (mixture of Universal Human Reference RNA with ERCC Exfold Spike-In Mix 1) with SENSE mRNA-Seq and conventional RNA-Seq.
Superior gene body coverage
- More evenly accumulated coverage of 3' and 5' transcript ends