困難檢體

swift biosciences
DNA 建庫專家

Formalin-fixed, Paraffin-embedded

Formalin-fixed, paraffin-embedding (FFPE) is a standard method for long term preservation of millions of archived human tissue samples. A challenge for next-generation sequencing (NGS) utilizing FFPE DNA is assessing low frequency somatic mutations that are key to cancer progression, offering desirable biomarkers for ultimately improving clinical outcomes for a wide spectrum of oncology-related diseases. The limited quantity and poor quality of DNA obtained from FFPE samples are major hurdles to the discovery of somatic mutations using NGS. Swift Biosciences offers sample preparation solutions to overcome these limitations for a variety of applications. Researchers typically experience a 20-25% failure rate in library preparation of FFPE samples. With our Accel-NGS® 2S Hyb DNA Library Kit and Accel-Amplicon™ Panels, end users can prepare high complexity NGS libraries with significantly lower failure rates. For extremely damaged DNA, the Accel-NGS 1S Plus DNA Library Kit offers the best solution to rescue precious samples.

SWIFT PRODUCT LINES COMPATIBLE WITH FFPE:

Accel-NGS 2S Hyb DNA Library Kit

The Swift advantage:

  • Compatible with multiple capture technology suitable for a diverse set of panels.
  • Highly efficient adapter ligation provides meaningful data from low input samples.
  • Exceptional library complexity results in more unique molecules available for capture.
  • No adapter titration or heat steps make the kit readily automatable.

Accel-Amplicon Panels

The Swift advantage:

  • Single-tube assay provides easy and consistent processing.
  • Ready-to-sequence libraries in 2 hours.
  • 10 ng input DNA required.
  • Average amplicon size of 138-149 bp is compatible with FFPE and cfDNA.
  • Continguous coverage provides complete coverage of desired regions.
  • Coverage uniformity > 95%.
  • On target > 95%.

Performance data

https://swiftbiosci.com/applications/ffpe/#exp-performance-data

Metagenomics

Next-generation sequencing (NGS) of DNA extracted from metagenomic samples has enabled the identification and characterization of low abundance and unculturable bacteria within environmental samples. However, several hurdles to obtaining an accurate representation of the microbial population in these samples still exist. Bias introduced by amplification strategies, whether MDA- or PCR-based, can skew the relative representation of bacterial and viral populations within the sample. Furthermore, the relative difficulty of extracting DNA from some bacterial species can limit or exclude representation of “hard-to-crack” microbes within a population. The Accel-NGS® 1S Plus and 2S DNA Library Preparation Kits allow users to enhance the accuracy of their metagenomics analyses using strategies that avoid amplification-introduced bias and enhance recovery of hard to extract microbial DNA.

SWIFT PRODUCT LINES COMPATIBLE WITH METAGENOMICS:

Accel-NGS 1S Plus DNA Library Kit

The Swift advantage:

  • Compatible with harsh DNA extraction methods to enable sequencing of difficult samples.
  • Direct adaptation of ssDNA converts all DNA within the sample into library.

Accel-NGS 2S DNA Library Kits

The Swift advantage:

  • Coverage of extreme base compositions allows detection of AT-/GC-rich microbes in samples.
  • Efficient library preparation reduces or eliminates bias-introducing amplification methods.

Performance data

https://swiftbiosci.com/applications/metagenomics/#exp-performance-data

Circulating, Cell-Free DNA

Circulating, cell-free nucleic acids have emerged as important biomarkers in cancer and clinical diagnostics and detection of various clinical conditions. Translational genomic research is utilizing cell-free DNA (cfDNA) to monitor personalized therapies based on specific tumor profiles. In order to research this valuable information, it is critical to be able to detect the low levels of cfDNA from whole blood samples.

SWIFT PRODUCT LINES COMPATIBLE WITH CIRCULATING, CELL-FREE DNA:

Accel-NGS 2S PCR-Free DNA Library Kit

The Swift advantage:

  • PCR-free libraries from 10 ng of cfDNA are compatible with limited input quantities.
  • High complexity libraries from less input enables deep sequencing for variant calling.
  • Minimal bias for AT-/GC-rich sequences provides accurate representation of inherently biased cfDNA.

Accel-Amplicon™ Panels

The Swift advantage:

  • 10 ng of input DNA required.
  • Amplicon sizes 120-160 bp are compatible with 165 bp cfDNA fragment sizes.
  • High coverage uniformity reads are evenly distributed across targets

Performance data

https://swiftbiosci.com/applications/circulating-cell-free-dna/#exp-performance-data